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Enter the PCR template right here (a number of templates are currently not supported). It is highly encouraged to work with refseq accession or GI (as opposed to the Uncooked DNA sequence) Each time achievable as This permits Primer-BLAST to raised discover the template and therefore complete greater primer specificity checking.
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Compared with oligo(dT) primers, these primers will not be template certain and are well suited for DNA synthesis working with Klenow fragments with DNA templates or for cDNA synthesis utilizing reverse transcriptase with mRNA templates.
two. If a repeat database from your similar organism is just not available, the databases from your closest mother or father of that organism while in the taxonomy tree will likely be chosen. One example is, the rodent repeat databases will probably be selected if "Mouse" is laid out in "Organism" industry.
A most of twenty assembly accessions are authorized. FASTA sequences are restricted to 300M. Notice the organism field is overlooked for custom databases. Enter sequence accession, FASTA sequence check here or assembly accession
In distinction to random primers, oligo (dT) primers are usually not suitable for degraded samples but are outstanding for constructing cDNA libraries and proposed for use with reverse transcriptases (RTs) as Superscript IV RT.
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Widespread sequences of Shorter DNA oligonucleotides are often made use of as primers for sequencing cloned genes (Sanger sequencing). Choose between our typically applied sequencing primers to initiate your sequence verification initiatives.
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In distinction to random primers, oligo (dT) primers usually are not suited to degraded samples but are superb for constructing cDNA libraries and advised for use with reverse transcriptases (RTs) as Superscript IV RT.